Imaging of the calibration sample for L-SPIMdoi:10.48788/DVUA/Q8I551DataverseUA2026-09-161Olifirov, Borys; Ustymenko, Valeriia, 2026, "Imaging of the calibration sample for L-SPIM", https://doi.org/10.48788/DVUA/Q8I551, DataverseUA, V1Imaging of the calibration sample for L-SPIMdoi:10.48788/DVUA/Q8I551Olifirov, BorysUstymenko, ValeriiaOlifirov, BorysFluorescence Imaging LaboratoryKyiv, UkraineMicroManagerpymmcore-plusРозпорядження НАН України №678 від 4.12.2024 р.DataverseUAFluorescence Imaging LaboratoryOlifirov, BorysFluorescence Imaging LaboratoryOlifirov, Borys2026-08-25Medicine, Health and Life SciencesPhysicsSPIMFluorescence microscopyTest acquisition using the prototype L-SPIM v0 setup. See detailed description in README.md. Sample details: - 0.2 μm yellow-green fluorescence beads. - Embended in 5% agarose.Fluorescence imaging dataPost-processed fluorescence imaging dataDataset structure: <br> - YG-02_x510_m545-40.tiff: original acquisition <br> - YG-02_x510_m545-40_crop.tiff: croped region of interest (ROI) <br> - YG-02_x510_m545-40_crop_interpolated.tiff: ROI with linear interpolation along z-axis to obtain isotropic pixel size (scale factor across z=625/493=1.27) <br> - psf_v0.tiff: distilled PSF <br> - report.png: PSF distillation and Gaussian fit results <br> <br> Images formatted as 16 bit grayscale uncompressed TIFFs, for viewing and editing use microscopic image viewers: ImageJ (https://imagej.net/ij/), Fiji (https://fiji.sc), napari (https://napari.org), Olympus FluoView, etc.For beads detection and distillation used <b>YG-02_x510_m545-40_crop_interpolated.tiff</b> file, individual detected beads avaliable in /beads folder. Analysis sript avaliable on GitHub: <b>https://github.com/wisstock/PSFDistiller.jl</b>psf_v0.tiffdistilled PSFimage/tiffREADME.mddetailed description of the datasettext/markdownreport.pngPSF distillation and Gaussian fit resultsimage/pngYG-02_x510_m545-40.tifimage/tiffYG-02_x510_m545-40_crop.tifcroped region of interest (ROI)image/tiffYG-02_x510_m545-40_crop_interpolated.tifROI with linear interpolation along z-axis to obtain isotropic pixel 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list of files and parameters.application/json