10.48788/DVUA/Q8I551Olifirov, BorysBorysOlifirov0000-0001-9915-7769Kyiv Academic University, Bogomoletz Institute of PhysiologyUstymenko, ValeriiaValeriiaUstymenko0009-0000-2241-435XBogomoletz Institute of Physiology, Kyiv Aviation InstituteImaging of the calibration sample for L-SPIMDataverseUA2026Medicine, Health and Life SciencesPhysicsSPIMFluorescence microscopyOlifirov, BorysBorysOlifirovKyiv Academic University, Bogomoletz Institute of PhysiologyFluorescence Imaging LaboratoryKyiv Academic University, Kyiv Aviation InstituteFluorescence Imaging LaboratoryKyiv Academic University, Kyiv Aviation InstituteOlifirov, BorysBorysOlifirovFluorescence Imaging LaboratoryKyiv Academic University, Kyiv Aviation Institute2026-08-252026-09-16Fluorescence imaging data117213432703012235359588124488490337928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928379283792837928285030application/jsontext/markdownimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/png1.0CC BY 4.0Test acquisition using the prototype L-SPIM v0 setup. See detailed description in README.md.
Sample details:
- 0.2 μm yellow-green fluorescence beads.
- Embended in 5% agarose.Dataset structure: <br>
- YG-02_x510_m545-40.tiff: original acquisition <br>
- YG-02_x510_m545-40_crop.tiff: croped region of interest (ROI) <br>
- YG-02_x510_m545-40_crop_interpolated.tiff: ROI with linear interpolation along z-axis to obtain isotropic pixel size (scale factor across z=625/493=1.27) <br>
- psf_v0.tiff: distilled PSF <br>
- report.png: PSF distillation and Gaussian fit results <br> <br>
Images formatted as 16 bit grayscale uncompressed TIFFs, for viewing and editing use microscopic image viewers: ImageJ (https://imagej.net/ij/), Fiji (https://fiji.sc), napari (https://napari.org), Olympus FluoView, etc.Kyiv, UkraineNational Academy of Sciences of UkraineРозпорядження НАН України №678 від 4.12.2024 р.