Imaging of the calibration sample for L-SPIM

Version 1.0
Imaging of the calibration sample for L-SPIM
Olifirov, Borys; Ustymenko, Valeriia, 2026, "Imaging of the calibration sample for L-SPIM", https://doi.org/10.48788/DVUA/Q8I551, DataverseUA, V1
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Description
Test acquisition using the prototype L-SPIM v0 setup. See detailed description in README.md. Sample details: - 0.2 μm yellow-green fluorescence beads. - Embended in 5% agarose. (2026-08-25)
Subject Medicine, Health and Life Sciences; Physics
Keyword SPIM
Notes Dataset structure:
- YG-02_x510_m545-40.tiff: original acquisition
- YG-02_x510_m545-40_crop.tiff: croped region of interest (ROI)
- YG-02_x510_m545-40_crop_interpolated.tiff: ROI with linear interpolation along z-axis to obtain isotropic pixel size (scale factor across z=625/493=1.27)
- psf_v0.tiff: distilled PSF
- report.png: PSF distillation and Gaussian fit results

Images formatted as 16 bit grayscale uncompressed TIFFs, for viewing and editing use microscopic image viewers: ImageJ (https://imagej.net/ij/), Fiji (https://fiji.sc), napari (https://napari.org), Olympus FluoView, etc.
License/Data Use Agreement

CC BY 4.0

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psf_v0.tiff
psf_v0.tiff
TIFF Image - 37.0 KB
Published Sep 16, 2026
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MD5: 79507fb702f2019675e3f8ac8dd7ec91
distilled PSF
README.md
Markdown Text - 1.3 KB
Published Sep 16, 2026
2 Downloads
MD5: 7742f889d354907e5fc0adb6b990a516
detailed description of the dataset
report.png
report.png
PNG Image - 278.3 KB
Published Sep 16, 2026
0 Downloads
MD5: 1a3ee66664ef103913b8b41b8ad1eb53
PSF distillation and Gaussian fit results
YG-02_x510_m545-40.tif
TIFF Image - 2.5 GB
Published Sep 16, 2026
0 Downloads
MD5: 76addf4cf27b2e2240bc1ea50958dea2
YG-02_x510_m545-40_crop.tif
TIFF Image - 34.3 MB
Published Sep 16, 2026
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MD5: dc3cb40f78e19d86f428d9fde935b2e9
croped region of interest (ROI)
YG-02_x510_m545-40_crop_interpolated.tif
TIFF Image - 42.8 MB
Published Sep 16, 2026
0 Downloads
MD5: e417149ff2ad2b127adc7f875abaee1f
ROI with linear interpolation along z-axis to obtain isotropic pixel size
bead_1-2.tiff
bead_1-2.tiff
beads/TIFF Image - 37.0 KB
Published Sep 16, 2026
0 Downloads
MD5: 95c223173fbacd092f58c70ba80a0eb0
bead_10-2.tiff
bead_10-2.tiff
beads/TIFF Image - 37.0 KB
Published Sep 16, 2026
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MD5: 8223e443db581bd222ad73c8c607fe98
bead_100-2.tiff
bead_100-2.tiff
beads/TIFF Image - 37.0 KB
Published Sep 16, 2026
0 Downloads
MD5: e9cad467b3ce4d8ccf2aea9e5a006e11
bead_101-2.tiff
bead_101-2.tiff
beads/TIFF Image - 37.0 KB
Published Sep 16, 2026
0 Downloads
MD5: 0d7769f2e143791079cb4b2cfb944bf2
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